DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA.
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DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA.
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The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.
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Knowledge of DNA sequencing sequences has become indispensable for basic biological research, DNA sequencing Genographic Projects and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics.
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Rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes, of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.
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DNA sequencing is the most efficient way to indirectly sequence RNA or proteins .
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DNA sequencing has become a key technology in many areas of biology and other sciences such as medicine, forensics, and anthropology.
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Information obtained using sequencing allows researchers to identify changes in genes and noncoding DNA, associations with diseases and phenotypes, and identify potential drug targets.
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Since DNA is an informative macromolecule in terms of transmission from one generation to another, DNA sequencing is used in evolutionary biology to study how different organisms are related and how they evolved.
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Viral DNA sequencing can be used to estimate when a viral outbreak began by using a molecular clock technique.
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DNA sequencing is being increasingly used to diagnose and treat rare diseases.
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DNA sequencing allows clinicians to identify genetic diseases, improve disease management, provide reproductive counseling, and more effective therapies.
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DNA sequencing testing has evolved tremendously in the last few decades to ultimately link a DNA sequencing print to what is under investigation.
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Testing DNA sequencing is a technique which can detect specific genomes in a DNA sequencing strand to produce a unique and individualized pattern.
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Canonical structure of DNA sequencing has four bases: thymine, adenine, cytosine, and guanine .
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DNA sequencing is the determination of the physical order of these bases in a molecule of DNA.
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Deoxyribonucleic acid was first discovered and isolated by Friedrich Miescher in 1869, but it remained under-studied for many decades because proteins, rather than DNA sequencing, were thought to hold the genetic blueprint to life.
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In 1953, James Watson and Francis Crick put forward their double-helix model of DNA sequencing, based on crystallized X-ray structures being studied by Rosalind Franklin.
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Foundation for DNA sequencing proteins was first laid by the work of Frederick Sanger who by 1955 had completed the sequence of all the amino acids in insulin, a small protein secreted by the pancreas.
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Traditional RNA sequencing methods require the creation of a cDNA molecule which must be sequenced.
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DNA polymerase catalysis and specific nucleotide labeling, both of which figure prominently in current sequencing schemes, were used to sequence the cohesive ends of lambda phage DNA.
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Several new methods for DNA sequencing were developed in the mid to late 1990s and were implemented in commercial DNA sequencers by 2000.
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Usually, this is accomplished by fragmenting the genome into small pieces, randomly sampling for a fragment, and DNA sequencing it using one of a variety of technologies, such as those described below.
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Sanger DNA sequencing is the method which prevailed from the 1980s until the mid-2000s.
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High-throughput sequencing, which includes next-generation "short-read" and third-generation "long-read" sequencing methods, applies to exome sequencing, genome sequencing, genome resequencing, transcriptome profiling, DNA-protein interactions, and epigenome characterization.
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The DNA sequencing is performed with use of unmodified polymerase and fluorescently labelled nucleotides flowing freely in the solution.
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Protein nanopore DNA sequencing utilizes membrane protein complexes such as a-hemolysin, MspA or CssG, which show great promise given their ability to distinguish between individual and groups of nucleotides.
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In contrast, solid-state nanopore DNA sequencing utilizes synthetic materials such as silicon nitride and aluminum oxide and it is preferred for its superior mechanical ability and thermal and chemical stability.
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The fabrication method is essential for this type of DNA sequencing given that the nanopore array can contain hundreds of pores with diameters smaller than eight nanometers.
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Polony sequencing method, developed in the laboratory of George M Church at Harvard, was among the first high-throughput sequencing systems and was used to sequence a full E coli genome in 2005.
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Parallelized version of pyroDNA sequencing was developed by 454 Life Sciences, which has since been acquired by Roche Diagnostics.
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The DNA sequencing machine contains many picoliter-volume wells each containing a single bead and DNA sequencing enzymes.
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Solexa, now part of Illumina, was founded by Shankar Balasubramanian and David Klenerman in 1998, and developed a DNA sequencing method based on reversible dye-terminators technology, and engineered polymerases.
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Unlike pyrosequencing, the DNA chains are extended one nucleotide at a time and image acquisition can be performed at a delayed moment, allowing for very large arrays of DNA colonies to be captured by sequential images taken from a single camera.
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DNA sequencing nanoballs are simply formed by denaturing double stranded, adapter ligated libraries and ligating the forward strand only to a splint oligonucleotide to form a ssDNA sequencing circle.
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DNA nanoball sequencing is a type of high throughput sequencing technology used to determine the entire genomic sequence of an organism.
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However, only short sequences of DNA sequencing are determined from each DNA sequencing nanoball which makes mapping the short reads to a reference genome difficult.
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DNA sequencing research, using microfluidics, has the ability to be applied to the sequencing of RNA, using similar droplet microfluidic techniques, such as the method, inDrops.
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The benefit of this DNA sequencing type is its ability to capture a large number of targets with a homogenous coverage.
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One end of DNA sequencing to be sequenced is attached to another bead, with both beads being placed in optical traps.
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Success of any DNA sequencing protocol relies upon the DNA or RNA sample extraction and preparation from the biological material of interest.
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