Flow cytometry is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles.
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14 Facts About Flow cytometry
2.
Flow cytometry is routinely used in basic research, clinical practice, and clinical trials.
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3.
Flow cytometry analyzer is an instrument that provides quantifiable data from a sample.
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4.
Original name of the fluorescence-based flow cytometry technology was "pulse cytophotometry", based on the first patent application on fluorescence-based flow cytometry.
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5.
Flow cytometry cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals.
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DNA6.
Spectral flow cytometry uses prisms or diffraction gratings to disperse the emitted light of a marker across a detector array.
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7.
Flow cytometry uses the light properties scattered from cells or particles for identification or quantitative measurement of physical properties.
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8.
Flow cytometry uses fluorescence as a quantitative tool; the utmost sensitivity of flow cytometry is unmatched by other fluorescent detection platforms such as confocal microscopy.
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9.
Mass Flow cytometry overcomes the fluorescent labeling limit by utilizing lanthanide isotopes attached to antibodies.
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10.
Mass cytometry is fundamentally different from flow cytometry: cells are introduced into a plasma, ionized, and associated isotopes are quantified via time-of-flight mass spectrometry.
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11.
Flow cytometry is widely applied to detect sperm cells abnormality associated with DNA fragmentation in male fertility assays.
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12.
In protein engineering, flow cytometry is used in conjunction with yeast display and bacterial display to identify cell surface-displayed protein variants with desired properties.
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13.
Flow cytometry allowed marine researchers to distinguish between dimly-fluorescing Prochlorococcus and heterotrophic microorganisms, a distinction that is difficult with microscopy-based assessments.
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14.
Flow cytometry has been used to measure genome sizes, or more precisely: the amount of DNA in a cell or nucleus.
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