11 Facts About Western blot

Western blot technique uses three elements to achieve its task of separating a specific protein from a complex: separation by size, transfer of protein to a solid support, and marking target protein using a primary and secondary antibody to visualize.

Name western blot is a play on the Southern blot, a technique for DNA detection named after its inventor, English biologist Edwin Southern.

Western blot is extensively used in biochemistry for the qualitative detection of single proteins and protein-modifications .

The western blot is routinely used for verification of protein production after cloning.

Confirmatory HIV test employs a western blot to detect anti-HIV antibody in a human serum sample.

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The western blot technique was used during the 2014 FIFA World Cup in the anti-doping campaign for that event.

Recent research utilizing the western blot technique showed an improved detection of EPO in blood and urine based on novel Velum SAR precast horizontal gels optimized for routine analysis.

Western blot method is composed of a gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide, followed by an electrophoretic transfer onto a membrane and an immunostaining procedure to visualize a certain protein on the blot membrane.

However, NC is brittle, and does not allow the Western blot to be used for re-probing, whereas PVDF membrane allows the Western blot to be re-probed.

The use of film for western blot detection is slowly disappearing because of non linearity of the image .

Fluorescently labeled probe is excited by light and the emission of the excitation is then detected by a photosensor such as a CCD camera equipped with appropriate emission filters which captures a digital image of the western blot and allows further data analysis such as molecular weight analysis and a quantitative western blot analysis.