1. In 1994, he moved to Juventus, where he spent majority of his career, winning 17 trophies; due to his success and performances with the club, Tacchinardi was one of the 50 Juventus players to have their names written inside the club's new home ground, the Juventus Stadium.
Alessio Tacchinardi spent two full seasons with the Bergamo-based club, where he made 9 league appearances, including his Serie A debut.
Alessio Tacchinardi scored two more goals in 13 UEFA Champions League appearances, a run in which Juventus reached the UEFA Champions League final, lost on penalties to Milan.
In July 2005, Alessio Tacchinardi was transferred to Villarreal CF who signed him on one-year loan from Juventus, following the appointment of Fabio Capello, who took over the managerial position from Marcello Lippi, and due in part to the purchases of Patrick Vieira and Federico Balzaretti.
Alessio Tacchinardi became an integral part of the starting XI for the club, helping the team to reach the UEFA Champions League semi-finals.
Alessio Tacchinardi returned to Juventus on 30 June 2006, but following the Calciopoli troubles he extended his loan for one more year, in July 2006.
Alessio Tacchinardi returned to Juventus again in the summer of 2007, and it was believed that he would remain at the club, following the appointment of Claudio Ranieri and the club's new start, however his contract was mutually terminated in August 2007, following certain unspecified disagreements with the club's new board of directors.
Alessio Tacchinardi signed a two-year deal with Brescia Calcio on 9 August 2007, and was a key attribute to the Serie B club's first team, appearing in all but 8 league games, and scored 11 goals.
Alessio Tacchinardi was not a regular player for the Squadra Azzurra, never playing in the final stages of a major tournament, in several cases due to injury, but because this was a period of many high quality Italian international midfielders.
Alessio Tacchinardi then took on more Serie D roles, in 2018 at Lecco and 2019 at Crema, with little success.
On 22 March 2021, Alessio Tacchinardi was appointed new head coach of Serie C club Fano.
On 20 June 2022, Alessio Tacchinardi agreed to return to work with Lecco in the Serie C league.
Alessio Tacchinardi was dismissed on 17 September 2022, after only three leagues games in charge of the club.
Alessio Tacchinardi primarily played as a central or defensive midfielder, although he was capable of playing as a right-sided winger or wing-back.
Alessio Tacchinardi was noted for his powerful and accurate long range shooting, as well as his striking ability from volleys, which saw him score several goals from distance during his time with Juventus.