Cell sorting is the process through which a particular cell type is separated from others contained in a sample on the basis of its physical or biological properties, such as size, morphological parameters, viability and both extracellular and intracellular protein expression.
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The homogeneous cell population obtained after sorting can be used for a variety of applications including research, diagnosis, and therapy.
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Fluorescence-Activated Cell Sorting, is known as flow cytometry cell sorting, or commonly known by the acronym FACS, which is a trademark of Beckton Dickinson and Company.
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Fluorescence activated cell sorting utilizes flow cytometry to separate cells based on morphological parameters and the expression of multiple extracellular and intracellular proteins.
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Fluorescence activated cell sorting has several systems that work together to achieve successful sorting of events of interest.
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Immunomagnetic cell sorting is known as immunomagnetic cell separation, immunomagnetic cell enrichment, or magnetic-activated cell sorting, and commonly known by the acronym MACS which is a trademark of Miltenyi GmbH.
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Immunomagnetic cell sorting is based on separation of beads passing a magnetic field.
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Immunomagnetic cell sorting provides a method for enriching a heterogeneous mixture of cells based on cell-surface protein expression.
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Immunomagnetic cell sorting therefore enables different strategies for positive enrichment or depletion of cells.
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Immunomagnetic cell sorting has shown to be beneficial when used with NPC cultures in particular, as it is easier to manage and causes minimal damage to live cells.
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Immunomagnetic cell sorting has been used in assistance with reproduction and retinal transplant treatment.
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Active microfluidic cell sorting requires similar cytometry instrumentation as fluorescence-activated cell sorting described above.
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Passive cell sorting uses the behavior of the fluid within the microchannels to alter and separate cells based on size and morphology.
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Newer cell separation technique employing antibodies is buoyancy-activated cell sorting is a separation technique in which microbubbles bind to cells through antibodies binding to the surface of cells.
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Single-cell sorting provides a method for sorting a heterogeneous mixture of cells based on intracellular and extracellular properties.
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